rho kinase inhibitor y27632 (Tocris)

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Tocris rho kinase inhibitor y27632
Rho Kinase Inhibitor Y27632, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 2615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rho+kinase+inhibitor/Y-27632+dihydrochloride/pmc13083800-209-43-47
Average 96 stars, based on 2615 article reviews

rho kinase inhibitor y27632 - by Bioz Stars, 2026-09

96/100 stars

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Injection:

Article Title: Matrix Stiffness Affects Spheroid Invasion, Collagen Remodeling, and Effective Reach of Stress into ECM
Article Snippet: .. After injection, spheroids were incubated with the appropriate medium, or medium containing 20 nM Rho kinase inhibitor (ROCK1/2 GSK 269962, Tocris, Bristol, UK, 4009) at 37 ◦C. .. As 4T1 tumor cells grew and migrated faster than SV80 fibroblast cells, 4T1 spheroids were incubated for 48 h, and SV80 spheroids for 72 h. All spheroids were fixed and stained with final concentrations of 2% formaldehyde (Sigma-Aldrich, 252549), 0.1% TritonX-100 (Sigma-Aldrich, T8787), 0.4 μg/mL Hoechst (Fisher Biotech, Wembley, Australia, 33258) and 0.05 μM AlexaFluor-488 Phalloidin (Invitrogen, Waltham, MA, USA, A12379) in PBS for 3 h at room temperature.

Incubation:

Single Cell:

Article Title: Goal-directed learning in cortical organoids.
Article Snippet: .. To generate cortical organoids we single cell dissociated ESCs using TrypLE Express Enzyme (ThermoFisher Scientific #12604021) for 5 minutes at 37 ◦ C and re-aggregated in lipidure-coated 96-well V-bottom plates at a density of 3,000 cells per aggregate, in 100 μL of mESC maintenance media supplemented with Rho Kinase Inhibitor (Y-27632, 10 μM, Tocris #1254) and 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma #ESG1107) (Day -1). .. After one day (Day 0), we replaced the medium with cortical differentiation medium containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), 10% Knockout Serum Replacement (Thermo Fisher Scientific # 10828028), 0.1 mM MEM Non-Essential Amino Acids (Thermo Fisher Scientific # 11140050), 1 mM Sodium Pyruvate (Millipore Sigma # S8636), 2 mM Glutamax supplement (Thermo Fisher Scientific # 35050061), 0.1 mM 2-Mercaptoethanol (Millipore Sigma # M3148), and 0.05 mg/ml Primocin (Invitrogen # ant-pm-05).

Recombinant:

Article Title: Label-free single cell phenotyping to determine tumor cell heterogeneity in pancreatic cancer in real-time.
Article Snippet: Tissue pellets were incubated for 3-10 min with red blood cell lysis buffer (#A1049201, Thermo Fisher Scientific) and further digested using TrypLE (#12604039, Thermo Fisher Scientific). .. Cell pellets were resuspended in 50 μl of Matrigel/well (#354230, Corning Life Sciences) and PDO medium (DMEM-F12 (#11320033, Thermo Fisher), 5mg/ml D-Glucose (#G8270, Sigma-Aldrich), 0.5% ITS Premix (#354350, Fisher Scientific), 5 nM 3,3,5-Triiodo-L-Thyronine (#T0821, Sigma Aldrich), 1 μM Dexamethasone (#D175, Sigma Aldrich), 100 ng/ml Cholera Toxin (#C9903, Sigma Aldrich), 1% penicillin/streptomycin (#15140122, Thermo Fisher Scientific), 5% NU-Serum IV (#355500, Fisher Scientific), 25 μg/ml Bovine Pituitary Extract (#P1167, Sigma Aldrich), 10 mM Nicotinamide (#N3376, Sigma Aldrich), 100 μg/ml Primocin (#ant-pm05, Invivogen), 0.5 μm A83-01 (#2939, Tocris), 10% RSPO1-conditioned medium (R-spondin-1 overexpressing cell line HEK293T, provided by the Hubrecht Institute (Uppsalalaan 8, 3584 CT Utrecht, Netherlands), 100 ng/ml Recombinant Human Heregulin-1 (#100-03, Peprotech), 10 μM Rho Kinase Inhibitor (#TB1254-GMP, Tocris) was added 10 min later. .. For passaging, media was aspirated and 250 μl Cell Recovery Solution (#11543560, Fisher Scientific) was added well for 5 min.

Article Title: Label-free single-cell phenotyping to determine tumor cell heterogeneity in pancreatic cancer in real time
Article Snippet: Tissue pellets were incubated for 3–10 minutes with RBC lysis buffer (A1049201, Thermo Fisher Scientific) and further digested using TrypLE (12604039, Thermo Fisher Scientific). .. Cell pellets were resuspended in 50 μL of Matrigel/well (354230, Corning Life Sciences), and PDO medium was added 10 minutes later after Matrigel was solidified: DMEM-F12; 5 mg/mL d -glucose (G8270, Sigma-Aldrich); 0.5% ITS Premix (354350, Thermo Fisher Scientific); 5 nM 3,3,5-triiodo- l -thyronine (T0821, Sigma-Aldrich); 1 μM dexamethasone (D175, Sigma-Aldrich); 100 ng/mL cholera toxin (C9903, Sigma-Aldrich); 1% penicillin/streptomycin (15140122, Thermo Fisher Scientific); 5% NU-Serum IV (355500, Thermo Fisher Scientific); 25 μg/mL bovine pituitary extract (P1167, Sigma-Aldrich); 10 mM nicotinamide (N3376, Sigma-Aldrich); 100 μg/mL Primocin (ant-pm05, InvivoGen); 0.5 μm A83-01 (2939, Tocris); 10% RSPO1-conditioned medium (R-spondin-1–overexpressing cell line HEK293T, provided by the Hubrecht Institute, Utrecht, the Netherlands); 100 ng/mL Recombinant Human Heregulin-1 (100-03, PeproTech); and 10 μM Rho Kinase Inhibitor (TB1254-GMP, Tocris). .. For passaging, media were aspirated, and 250 μL Cell Recovery Solution (11543560, Thermo Fisher Scientific) was added to wells for 5 minutes.

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